Cellular Therapy Laboratory

Our cellular therapy laboratory is dedicated to providing blood and marrow transplants for patients undergoing treatment for certain cancers. These products provide hematopoietic progenitor cells (HPCs), which are the precursors to red blood cells, white blood cells and platelets.

In the case of patients being infused with their own (autologous) HPCs, Blood Systems Laboratories handles the processing, cryopreservation and storage of the cells for transplant following collection, and then the delivery and bedside preparation when the patient is ready to be reinfused with the healthy cells. When patients require HPCs from a donor (allogeneic), Blood Systems Laboratories performs HLA (human leukocyte antigen) matches on relatives or on unrelated donors who are registered through the National Marrow Donor Program®.

For the hospital oncology and transplant programs treating these patients, using Blood Systems Laboratories avoids the burden of obtaining complex new certifications and the expense of outside testing. The service is all-inclusive, giving clinical teams the continuity of providers while offering peace of mind for their patients that their own or their donor’s cells are treated with care and respect through every stage in their treatment process.

The cellular therapy laboratory at Blood Systems Laboratories is accredited by AABB and Foundation for the Accreditation of Cellular Therapy (FACT) and is an FDA-registered tissue bank.

For clinical consultations, please call (602) 343-7103.

Contact us to discuss our cellular therapy services.

View sample of the CTL Requisition Form (HPP 163).

View sample of the CTL Requisition Form (HPP 203).

Laboratory Category Test Name Description Turn Around Times Sample, Packaging & Shipping Requirements CPT Code Form
CTL Autologous & Allogeneic Stem Cells ABO Incompatibility Processing – Plasma Depletion

Removal of plasma

NA NA 38214 CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells ABO Incompatibility Processing – Red Cell Depletion

Removal of red cells from product received

NA NA 38212 CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow (Autologous & Allogeneic)

Processing, splitting and labeling additional bags from original collection. Includes testing for CD34, CD3, Total Nucleated Count, Manual Differential, Sterility, Viability, Infectious Disease Markers and cryopreservation of up to 6 bags for storage up to 36 months.

NA NA 38207, 85007, 85025, 86359, 86367, 87040x2, 88237 CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells Base Lab Processing of Pheripheral Blood Progenitor Cells and Bone Marrow After Hours

Base lab processing from 2300 to 0600, weekends and holidays

NA NA NA CTL Requisition Form HPP 163
CTL Blood & Marrow Transplant CD34 Enumeration with Viability

Determination of absolute count, percentage, and viability of CD34 progenitor cells in peripheral blood, pheresis product or bone marrow by flow cytometry

2 hours from sample receipt, when lab notified in advance Samples must be received within 24 hours of collection. EDTA 3mL of anticoagulated whole blood; EDTA 0.5mL of pheresis unit, cord blood or bone marrow anticoagulated 86367, 88184, 88185x2, 88187 CTL Requisition Form HPP 203
CTL Processing Tests Colony Forming Units (CFU)

Culture of hematopoietic progenitor cells (HPCs) including erythroid, myeloid and megakaryocytic

14 to 16 days Cryovial, product bag, cell culture medium, eg RPMI, IMDM 88237 CTL Requisition Form HPP 203
CTL Processing Tests Complete Blood Count

Total nucleated cell count, hematocrit and a breakout of mononuclear cell and granulocyte percentages

24 to 48 hours Sample size is 0.5mL, red top tube, shipped at 1-10 C 85025 CTL Requisition Form HPP 203
CTL Autologous & Allogeneic Stem Cells Cord Blood Dilution – No Wash (with pre & post CD34)

Dilution of DMSO from cord product with CD 34 testing

NA NA 86367 CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells Cord Blood Dilution – No Wash (without CD34)

Dilution of DMSO from cord product

NA NA NA CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells Fresh Allogeneic Processing without cryopreservation, Bone Marrow or Pheripheral Blood Progenitor Cells

Base lab processing services and testing for fresh products listed

NA NA 85007, 85025, 86359, 86367, 87040x2, 88237 CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells Labeling Extra Bags after the 6th

Utilizing extra bags during processing for product storage, including labeling

NA NA NA CTL Requisition Form HPP 163
CTL Processing Tests Manual Differential – on product received at BSL

Manual white blood cell count differential

24 hours Cryovial, product bag, EDTA tube 85007 CTL Requisition Form HPP 203
CTL Autologous & Allogeneic Stem Cells Sepax Cord Blood Product Wash (per bag)

Automated cord blood washing in a functionally closed system

NA NA 38209 CTL Requisition Form HPP 163
CTL Processing Tests Sterility

Bacterial and fungal cultures on product

14 to 16 days Cryovial, product bag, tissue transport medium 87040x2 CTL Requisition Form HPP 203
CTL Storage with or without Cryopreservation Storage of Peripheral Blood Progenitor Cells and Bone Marrow

Stem Cell Storage per bag with no processing or cryopreservation up to 36 months

NA NA NA CTL Requisition Form HPP 163
CTL Storage with or without Cryopreservation Storage of Peripheral Blood Progenitor Cells and Bone Marrow with cryopreservation

Cryopreservation for each bag over 6 with storage up to 36 months

NA NA 38207 CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells T-Cell Therapeutic Processing

Base lab processing services and testing and up to 36 months of cryopreservation for up to 6 bags

NA NA 38207, 85007, 85025, 86359, 86367, 87040x2, 88237 CTL Requisition Form HPP 163
CTL Processing Tests Trypan Blue Viability

Simple dye exclusion test to measure the percentage of viable white cells

2 hours; M-F 8am-8pm 0.5mL of anticoagulant HPC product, shipped at 1 -10 C 85032 CTL Requisition Form HPP 203
CTL Autologous & Allogeneic Stem Cells Volume Reduction

Reduce plasma as required for cryopreservation purposes

NA NA 38214 CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells Wash – Manual (with pre & post CD34)

Thawing of previously frozen harvest with washing and CD34 testing

NA NA 38209, 86367 CTL Requisition Form HPP 163
CTL Autologous & Allogeneic Stem Cells Wash – Manual (without CD34)

Thawing of previously frozen harvest with washing

NA NA 38209 CTL Requisition Form HPP 163

Blood Systems and its divisions and affiliates provide comprehensive transfusion medicine services. These include: BioCARE • Blood Services (Blood Centers of the Pacific, BloodSource, Bonfils Blood Center, Community Blood Services, Inland Northwest Blood Center, Lifeblood, LifeShare, LifeStream, United Blood Services) • Blood Systems Laboratories • Blood Systems Research Institute • Creative Testing Solutions

BSI-K-L1

Blood Systems
National Office
6210 E. Oak Street
Scottsdale, AZ 85257
(800) 288-2199