Sample Requirements

A. Minimum sample collection and storage requirements for CHILDREN:

1. Infant (<1 year): 3 mL EDTA (purple top) anticoagulated whole blood for Class I, II
2. Child (1 year-10 years): 5 mL EDTA for Class I, II
3. Child (>10 years): 10 mL EDTA for Class I, II
4. Contact the HLA Laboratory when the WBC count is < 5.0 X 10³ for required sample amount.
5. Samples may be refrigerated (4°C) or stored at room temperature.

B. Sample collection and storage requirements for ADULTS:

1. HLA Class I (A,B,C,) or Class II (DR,DQ, DP) Typing
   
   1. Specimen: Collect 20 mL EDTA, mix thoroughly. When Class I and Class II are requested 20 mL EDTA will be sufficient.
      
   Collect 40 - 60 mL EDTA when the WBC count
   is < 5.0 X 10³. Contact the HLA laboratoy for assistance in determining required sample amount.
   
   2. Storage temperature: Refrigerated (4°C) or room temperature.
   
   
   
2. B-27 Typing
   
   1. Specimen: Collect 5 mL EDTA, mix thoroughly.
   2. Storage temperature: Refrigerated (4°C) or room temperature.
      Note: ACD may be substituted for EDTA anticoagulated samples for DNA molecular testing.
      
      
3. Antibody Screen
   
   1. Specimen: Collect 10 mL whole blood in a clot tube (red top).
   2. Storage temperature: Store and transport at 4°C. If not shipped within 48 hours of collection, separate and freeze serum. Send on ice.
      
      
4. HLA Crossmatch
   
   1. Recipient Specimen: Collect 10 mL whole blood in a clot tube (red top).
   2. Recipient Specimen Storage Temperature: Store and transport at 4°C. If not sent with in 48 hours of collection, separate and freeze the serum. Send on ice.
   3. Donor Specimen: Collect 30 mL ACD (yellow top) anticoagulated whole blood.
   4. Donor Specimen Storage Temperature: Store and transport at room temperature.